Objectives
Specimens: microscope slides
Other specimens
IntroductionFungi are among our closest relatives outside the animal kingdom. Like animals, they are heterotrophs. Their bodies are specialized for absorbing their food, and, like animals, they do much of their digestion outside their cells. They secrete enzymes to break down food so it can be absorbed into the cells. However, unlike animals, they don’t swim, walk, or fly to find their food. Fungi move into new food sources by growing or by the passive dispersal of nonmotile spores. The basic body design of fungi is simple, but highly functional: it’s all about absorbing food molecules from the environment. Show
Like plants, fungi have cell walls. However, the cell wall material of fungi is completely different from that of plants. Fungi have cell walls made of chitin, the same tough polysaccharide that makes up insect exoskeletons. Plants have cell walls made of cellulose, a different polysaccharide. Multicellular fungi are typically composed of long, thin filaments called hyphae; the whole body of many hyphae is called a mycelium. Multicellular fungi are always composed of hyphae; even mushrooms, which are the reproductive structures of underground fungi, are composed of these threadlike filaments of cells. Fungal mycelia have enormous surface area for absorption. Also, the hyphae can rapidly grow through soil as they move toward new food sources. Some fungi are unicellular; these are called yeasts. Yeasts are described in more detail below. SaccharomycesSaccharomyces is the common yeast often used in making wine, beer, and bread. The term "yeast" simply refers to fungi that grow as single, roundish cells and don't form hyphae. Yeast is not a taxonomic group; it's a description of a body type. These eukaryotic cells contain a nucleus (stained dark in this image) and a central vacuole (light staining), as well as other internal membrane-bound structures. AspergillusThis is a typical mold, showing asexual spore formation. Various species of Aspergillus are used commercially, for example in the production of citric acid or digesting the starch in rice as a step in making sake. Some species of Aspergillus occasionally act as human pathogens. Aspergillus is a typical mold, a mold is a fungus with a body composed of thin, stringy hyphae. The whole body of connected hyphae is called a mycelium. The mycelium is haploid, except for reproductive structures. Aspergillus is an ascomycete (phylum Ascomycota). The sporangia (called conidiophores) produce asexual spores called conidia. Conidia germinate to form new haploid mycelia for growth or specialized hyphae that perform plasmogamy. Compare this slide to the life cycle of Neurospora (fig. 31.16 in Campbell), but our slides may contain only the asexual sporangia. Molds can spread rapidly because their thin hyphae penetrate into new food sources (rotting fruit, for example) and can grow very rapidly. Molds can also produce huge numbers of asexual spores via mitosis. Molds also reproduce sexually, but this is much less common than asexual spore formation. RhizopusRhizopus is another common mold. Like Aspergillus, it typically makes a huge number of asexual spores and undergoes sexual reproduction less frequently. This image shows sporangia, which produce spores. These sporangia could be produced either sexually or asexually. During sexual reproduction, two haploid hyphae from different parents perform plasmogamy, joining together, producing a heterokaryotic cell. This multinucleate heterokaryotic cell forms a zygosporangium. Pairs of nuclei (one from each parent) perform karyogamy in the zygosporangium, fusing to form a diploid nucleus. These diploid nuclei are zygotes; they immediately undergo meiosis to begin producing haploid sexual spores. A sporangium sprouts out of the zygosporangium to release these spores. If you're looking at this slide, how would you know whether you are looking at an asexual sporangium or a sexual sporangium (zygosporangium)? Which do you think is produced more often? CoprinusCoprinus is in the phylum Basidiomycota, the phylum that makes mushrooms. Like many mushroom-forming fungi, Coprinus may have very large haploid mycelia underground, while occasionally forming heterokaryotic hyphae that grow into mushrooms above ground. The main body of the mushroom is heterokaryotic. In this case, each cell has two different nuclei in each cell; this kind of heterokaryotic cell is called dikaryotic. The main umbrella-shaped part is sometimes called the cap. Under the cap there are gills. (The gills have nothing to do with gas exchange; they're called gills because they look like fish gills.) Some of the dikaryotic cells on the gills undergo karyogamy, fusing their two different haploid nuclei to make a diploid zygote. As with all fungi, the zygote then performs meiosis, making four haploid cells. These haploid cells turn into spores. These images show the gills in a cross-section of the mushroom at different magnifications. This magnified view gives you a better view of the spores. Compare this to the life cycle diagram in Campbell. Are the cells you see here haploid, diploid or heterokaryotic? PezizaPeziza is a cup fungus in the phylum Ascomycotes. Like a mushroom (phylum Basidiomycota), Peziza produces above-ground heterokarkyotic reproductive structures that come from an underground mycelium. One way that Peziza differs from mushrooms is that it produces spores on top of its cup, not underneath like a mushroom. In cross-section, Peziza shows a mycelium constructed of loose hyphae underneath, with tightly packed spore-producing structures (called asci) on top. In this image, each ascus is a long, narrow cell with eight spores inside. The ascus begins as a single dikarkyotic cell. The two nuclei fuse, forming a single diploid nucleus; this is called karyogamy. The diploid nucleus is called a zygote. The zygote undergoes meiosis, producing four haploid nuclei. Each of these haploid nuclei divides once (via mitosis). The end result is eight haploid nuclei in one ascus; these nuclei form new walls and turn into spores. In this image, you can see spores at different stages of maturation. LichensA lichen is two different organisms living in a close symbiotic association. One partner is a fungus, which forms a tough, leathery coating that provides a protected space inside. The fungal cells can tolerate harsh, dry conditions, but they cannot produce food on their own. The other partner is an alga -- a unicellular photosynthetic organism. The algae thrive in the protected environment created by the fungal mycelium. The algae perform photosynthesis, making the sugars that can be used as energy by both the algae and the fungus. ReviewStudy QuestionsYou don’t need to turn in answers to these questions. However, you may want to think about them to help you prepare for the next lab exam.
Terms & Concepts to Remember
References & further readingCampbell Biology, Chapter 31: Fungi. In particular, study figure 31.12 (The life cycle of Rhizopus) and figure 31.18 (The life cycle of a mushroom-forming basidiomycete). What is the life cycle of a fungus?The life cycle of fungi can follow many different patterns. For most of the molds indoors, fungi are considered to go through a four-stage life cycle: spore, germ, hypha, mature mycelium. Brundrett (1990) showed the same cycle pattern using an alternative diagram of the developmental stages of a mould.
What does it mean if fungal hyphae is Heterokaryotic?Hyphae from opposite mating types produce structures that contain several haploid nuclei. Fusion of two of these structures from opposite mating types results in a heterokaryotic zygosporangium.
What stage is a Basidiomycete life cycle?Figure 8: Basidiomycete life cycle. The lifecycle of a basidiomycete has alternate generations with haploid and dikaryotic mycelia. Haploid primary mycelia fuse to form a dikaryotic secondary mycelium, which is the dominant stage of the life cycle, and produces the basidiocarp.
Which the following types of fungi have no Heterokaryotic phase in their life cycle?Parasexual fungi do not form a heterokaryotic stage, whereas sexually reproducing fungi do form a heterokaryotic stage. Crossing over occurs during mitosis in parasexual fungi and meiosis in sexually reproducing fungi.
|